Advanced Technologies for Protein Complex Production and Characterization

Advanced Technologies for Protein Complex Production and Characterization

Vega, M. Cristina

Springer International Publishing AG

05/2018

382

Mole

Inglês

9783319800943

15 a 20 dias

7409

Descrição não disponível.
Protein complex production from the drug discovery standpoint.- Choose a suitable expression host: a survey of available protein production platforms.- ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts.- Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput.- Membrane protein production in E. coli for applications in drug discovery.- Cell-free Synthesis of Macromolecular Complexes.- A Bacillus megaterium system for the production of recombinant proteins and protein complexes.- Protein complex production in alternative prokaryotic hosts.- Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts.- Leishmania tarentolae for the Production of Multi-Subunit Complexes.- Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes.- Fundamentals of baculovirus expression and applications.- The MultiBac Baculovirus / Insect Cell Expression Vector System for Producing Complex Protein Biologics.- Fundamentals of expression in mammalian cells.- Assembling multi-subunit complexes using mammalian expression.- Microalgae as solar-powered protein factories.- Strategies and methodologies for the co-expression of multiple proteins in plants.- Transient expression systems in plants-potentialities and constraints.- Complex reconstitution from individual protein modules.- Structural reconstruction of protein-protein complexes involved in intracellular signaling.- The use of small-angle scattering for the characterization of multi subunit complexes.- Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems.
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Acid complexes;Protein complexes;Expression systems;Eukaryotic expression systems;Structural and biophysical characterization;Protein Structure;Systems Biology